{"id":21932,"date":"2023-05-31T16:03:45","date_gmt":"2023-05-31T08:03:45","guid":{"rendered":"http:\/\/www.chonghwa.edu.my\/v2\/?p=15657"},"modified":"2023-05-31T16:03:45","modified_gmt":"2023-05-31T08:03:45","slug":"%e3%80%90%e6%96%b0%e5%8a%a0%e5%9d%a1%e5%9b%bd%e7%ab%8b%e5%a4%a7%e5%ad%a6%e7%94%9f%e7%89%a9%e5%b7%a5%e4%bd%9c%e5%9d%8a%e3%80%91","status":"publish","type":"post","link":"https:\/\/chonghwa.info\/v3\/2023\/05\/31\/%e3%80%90%e6%96%b0%e5%8a%a0%e5%9d%a1%e5%9b%bd%e7%ab%8b%e5%a4%a7%e5%ad%a6%e7%94%9f%e7%89%a9%e5%b7%a5%e4%bd%9c%e5%9d%8a%e3%80%91\/","title":{"rendered":"\u3010\u65b0\u52a0\u5761\u56fd\u7acb\u5927\u5b66\u751f\u7269\u5de5\u4f5c\u574a\u3011"},"content":{"rendered":"

\n\t\uff08\u56fe\u4e0a\uff09\u7531\u8d22\u653f\u738b\u57f9\u8363\uff08\u5de67\uff09\u79fb\u4ea4\u7d00\u5ff5\u54c1\u4e88\u4e18\u624d\u826f\u6559\u6388\uff08\u5de68\uff09\uff0c\u5de6\u8d77\uff1a\u6797\u5a49\u541b\u751f\u7269\u8001\u5e08\u3001\u8c2d\u6167\u5170\u526f\u6559\u52a1\u4e3b\u4efb\u517c\u751f\u7269\u79d1\u4e3b\u4efb\u3001\u738b\u4fca\u5357\u6559\u6388\u3001\u90d1\u70b3\u4eca\u6559\u6388\u3001\u5434\u5c0f\u71d5\u7f72\u7406\u6821\u957f\u3001\u76d1\u5b66\u9648\u6cbb\u9274\u3002\u53f3\u8d77\uff1a\u674e\u4ee3\u82b9\u6559\u6388\u3001\u9093\u5b87\u822a\u5728\u8bfb\u535a\u58eb\u3001\u4f59\u58ee\u4e3d\u5c0f\u59d0\u3001\u8096\u5a67\u8587\u5c0f\u59d0\u3001\u5434\u91d1\u7089\u535a\u58eb\u3001\u6797\u9752\u677e\u535a\u58eb\u3001\u5218\u6d77\u71d5\u6559\u6388\u3002<\/span><\/strong>\n<\/p>\n

\n\t(\u5c45\u92ae31\u8baf) \u5c45\u92ae\u4e2d\u534e\u4e2d\u5b66\u6559\u52a1\u5904\u4e8e\u4e94\u6708\u4e09\u5341\u65e5\u5728\u8be5\u6821\u4e3e\u529e2023\u5e74“\u65b0\u52a0\u5761\u56fd\u7acb\u5927\u5b66\u751f\u7269\u5de5\u4f5c\u574aNUS BIOLOGY WORKSHOP”\uff0c\u53c2\u4e0e\u5b66\u6821\u6709\u5c45\u92ae\u4e2d\u534e\u4e2d\u5b66\u4e0e\u6c38\u5e73\u4e2d\u5b66\uff0c\u5171\u8ba1\u5b66\u751f98\u4eba\uff0c\u751f\u7269\u5de5\u4f5c\u574a\u7684\u5185\u5bb9\u5305\u62ec\u4e13\u9898\u8bb2\u5ea7\u548cPCR\u6280\u672f\u5b9e\u9a8c\u3002<\/span>\n<\/p>\n

\n\t\u5c45\u92ae\u4e2d\u534e\u4e2d\u5b66\u7f72\u7406\u6821\u957f\u5434\u5c0f\u71d5\u8868\u793a\u8fd9\u6b21\u751f\u7269\u5de5\u4f5c\u574a\u7684\u4e3e\u529e\u5bf9\u92ae\u4e2d\u6765\u8bf4<\/span>\u662f\u4e00\u4e2a\u91cd\u8981\u7684\u91cc\u7a0b\u7891,\u751f\u7269\u79d1\u5b66\u7684\u53d1\u5c55\u548c\u5e94\u7528\u662f\u4ee5\u524d\u6240\u672a\u6709\u7684\u901f\u5ea6\u548c<\/span>\u5e7f\u5ea6\u5f71\u54cd\u7740\u6211\u4eec\u7684\u751f\u6d3b\u548c\u793e\u4f1a\u3002\u901a\u8fc7\u8fd9\u4e00\u6b21\u65b0\u52a0\u5761\u56fd\u7acb\u5927\u5b66\u3001<\/span>\u6c38\u5e73\u4e2d\u5b66\u548c\u5c45\u92ae\u4e2d\u534e\u4e2d\u5b66\u7684\u4e92\u52a8\u3001\u5b66\u4e60\u3001\u4ea4\u6d41\u548c\u5206\u4eab\u4e4b\u540e\uff0c<\/span>\u5b66\u751f\u4eec\u5fc5\u5b9a\u53ef\u4ee5\u66f4\u4e86\u89e3\u751f\u7269\u5b66\u79d1\u7684\u53d1\u5c55\u3001<\/span>\u5b9e\u9a8c\u7684\u8fdb\u884c\u4ee5\u53ca\u73af\u5883\u536b\u751f\u7684\u6c38\u7eed\u53d1\u5c55\u3002<\/span>\n<\/p>\n

\n\t\u65b0\u52a0\u5761\u56fd\u7acb\u5927\u5b66\u751f\u7269\u79d1\u5b66\u7cfb(\u7b80\u79f0\u56fd\u5927\u751f\u7269\u7cfb)\u5c06\u5728\u9a6c\u6765\u897f\u4e9a\u7684\u534e\u6587<\/span>\u72ec\u7acb\u4e2d\u5b66\u4e3e\u529e\u751f\u7269\u5de5\u4f5c\u574a,\u91cd\u70b9\u4ecb\u7ecd\u76ee\u524d\u751f\u547d\u79d1\u5b66\u7684\u8fdb\u5c55\u72b6\u51b5\u548c\u4e00\u4e9b<\/span>\u57fa\u672c\u7684\u5206\u5b50\u751f\u7269\u5b66\u7684\u5b9e\u9a8c\u64cd\u4f5c\u3002\u5e0c\u671b\u80fd\u52a0\u5f3a\u4e2d\u5b66\u751f(\u9ad8\u4e00\u81f3\u9ad8\u4e09)\u5bf9<\/span>\u751f\u547d\u79d1\u5b66\u7684\u8ba4\u8bc6\u53ca\u5174\u8da3\uff0c\u4e5f\u9f13\u52b1\u540c\u5b66\u4eec\u6765\u56fd\u5927\u5b66\u4e60\u3002\u56fd\u5927\u751f\u7269\u7cfb\u6709\u767e<\/span>\u5e74\u5386\u53f2,\u6559\u5b66\u548c\u79d1\u7814\u6c34\u5e73\u4e5f\u662f\u4e16\u754c\u4e00\u6d41\u3002\u56fd\u5927\u975e\u5e38\u91cd\u89c6\u79d1\u666e\u6559\u80b2\uff0c<\/span>\u4e3e\u529e\u4e86\u4f17\u591a\u7684\u79d1\u5b66\u8bb2\u5ea7\u548c\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u8bfe\u7a0b\u4ee5\u9f13\u52b1\u4e2d\u5b66\u751f\u6765\u56fd\u5927\u5b9e<\/span>\u4e60\u3002\u56fd\u5927\u751f\u7269\u7cfb\u9664\u4e86\u548c\u6b27\u7f8e\u3001\u4e2d\u56fd\u7684\u5927\u5b66\u6709\u5e7f\u6cdb\u6df1\u539a\u7684\u79d1\u7814\u5408\u4f5c\u4e4b\u5916<\/span>,\u4e5f\u5f3a\u8c03\u548c\u4e1c\u5357\u4e9a\u9ad8\u6821\u5efa\u7acb\u826f\u597d\u7684\u5408\u4f5c\u5173\u7cfb\u3002<\/span>\n<\/p>\n

\n\t\u5728\u8fd9\u6b21\u8bb2\u5ea7\u5185\u5bb9\u65b9\u9762,\u5305\u542b\u751f\u7269\u5f71\u50cf\uff08\u90d1\u70b3\u4eca\u6559\u6388\uff09\u3001\u5168\u7403\u6696\u5316\u8bfe\u9898<\/span>(\u738b\u4fca\u5357\u6559\u6388)\u3001\u513f\u7ae5\u9057\u4f20\u75c5\u7684\u75c5\u7406(\u5218\u6d77\u71d5\u6559\u6388)\u4ee5\u53ca\u751f\u7269\u591a\u6837\u6027<\/span>\uff08\u674e\u4ee3\u82b9\u6559\u6388\uff09\u3002\u90d1\u70b3\u4eca\u6559\u6388\u662f\u7535\u5b50\u663e\u5fae\u955c\u7684\u5148\u9a71\uff0c<\/span>\u738b\u4fca\u5357\u6559\u6388\u66f4\u662f\u8054\u5408\u56fd\u7684\u6c34\u8d44\u6e90\u4e13\u5bb6,\u66fe\u62c5\u4efb\u65b0\u52a0\u5761\u65b0\u751f\u6c34\u79d1\u5b66\u59d4\u5458<\/span>\u4f1a\u7684\u4e3b\u5e2d\u3002\u5218\u6d77\u71d5\u6559\u6388\u662f\u7af9\u811a\u5987\u5e7c\u533b\u9662\u7684\u9996\u5e2d\u79d1\u5b66\u5bb6,Duke-<\/span>NUS\u533b\u5b66\u9662\u7684\u6559\u6388,\u957f\u671f\u4ece\u4e8b\u513f\u7ae5\u9057\u4f20\u75c5\u7684\u7814\u7a76\u5de5\u4f5c\u3002<\/span>\u674e\u4ee3\u82b9\u6559\u6388\u5bf9\u8df3\u86db\u7684\u751f\u6001\u6709\u6df1\u5ea6\u7684\u7814\u7a76\u3002<\/span>\n<\/p>\n

\n\t\u9886\u961f\u961f\u957f\u4e18\u624d\u826f\u6559\u6388\u8868\u793a2023\u5e74\u7684“\u751f\u7269\u5de5\u4f5c\u574a”<\/span>\u5c06\u91cd\u70b9\u653e\u5728\u67d4\u4f5b\u548c\u5409\u9686\u5761\u7684\u91cd\u594c\u72ec\u4e2d\u8fdb\u884c\u3002\u4e18\u624d\u826f\u6559\u6388\u6bd5\u4e1a\u4e8e\u6021\u4fdd\u80b2<\/span>\u624d\u4e2d\u5b66\uff0c\u65b0\u52a0\u5761\u5357\u6d0b\u5927\u5b66\uff0c\u82f1\u5c5e\u54e5\u4f26\u6bd4\u4e9a\u5927\u5b66(\u535a\u58eb)\uff0c<\/span>\u66fe\u7ecf\u5728\u8036\u9c81\u5927\u5b66\u505a\u535a\u58eb\u540e\uff0c<\/span>\u62c5\u4efb\u8fc7\u52a0\u62ff\u5927\u7ebd\u82ac\u5170\u5927\u5b66\u548c\u591a\u4f26\u591a\u5927\u5b66\u6559\u6388\u3001<\/span>\u65b0\u52a0\u5761\u56fd\u7acb\u5927\u5b66\u751f\u7269\u79d1\u5b66\u7cfb\u7cfb\u4e3b\u4efb\u548c\u529b\u5b66\u751f\u7269\u5b66\u7814\u7a76\u6240\u526f\u6240\u957f\u3002<\/span>\u4e18\u6559\u6388\u79ef\u6781\u63a8\u884c\u751f\u7269\u5de5\u4f5c\u574a\u4e5f\u662f\u996e\u6c34\u601d\u6e90\uff0c\u5e0c\u671b\u4e3a\u534e\u6821\u5c3d\u7ef5\u8584\u4e4b\u529b\u3002<\/span>\n<\/p>\n

\n\t\u5728\u5b9e\u9a8c\u65b9\u9762,\u56e2\u961f\u5c06\u4e3a\u5b66\u751f\u4eec\u51c6\u5907\u5206\u5b50\u5b9e\u9a8c\u7684\u64cd\u4f5c\u5982DNA\u7684\u63d0\u53d6\uff0c<\/span>\u7535\u6cf3\u548cPCR\u64cd\u4f5c\u539f\u7406\uff0c\u4f7f\u540c\u5b66\u4eec\u6709\u52a8\u624b\u7684\u673a\u4f1a\u548c\u5174\u8da3\u3002\u7531\u591a\u4f4d\u79d1\u7814<\/span>\u4eba\u58eb\u5373\u5434\u91d1\u7089\u535a\u58eb\u3001\u6797\u9752\u677e\u535a\u58eb\u3001\u9093\u5b87\u822a\u5728\u8bfb\u535a\u58eb\u3001\u4f59\u58ee\u4e3d\u3001<\/span>\u8096\u5a67\u8587\u7b49\u4eba\u7684\u534f\u52a9\u4e0b\uff0c\u5e26\u9886\u540c\u5b66\u4eec\u4eb2\u81ea\u52a8\u624b\u505a\u5b9e\u9a8c\u3002<\/span>\u5b9e\u9a8c\u9879\u76ee\u6709\u5b66\u4e60\u4f7f\u7528\u5fae\u91cf\u79fb\u6db2\u5668\uff08\u5b66\u751f\u64cd\u4f5c\uff09\u3001\u8bbe\u7f6ePCR\u53cd\u5e94(\u6f14<\/span>\u793a\u548c\u8bb2\u89e3)\u3001\u7535\u6cf3\uff08\u5b66\u751f\u64cd\u4f5c\uff09\uff0c\u4ece\u9999\u8549\u4e2d\u63d0\u53d6DNA\uff08\u5b66\u751f\u64cd\u4f5c\uff09<\/span>\u7b49\u3002<\/span>\n<\/p>\n

\n\tPCR\u662f\u4e00\u79cd\u901a\u8fc7\u70ed\u5faa\u73af\u53cd\u5e94\u6765\u6269\u589eDNA\u7684\u6280\u672f\uff0c<\/span>\u70ed\u5faa\u73af\u662f\u6307\u5728\u56fa\u5b9a\u7684\u65f6\u95f4\u95f4\u9694\u5185\u6e29\u5ea6\u53d8\u5316\u7684\u5faa\u73af\u3002\u91c7\u7528\u70ed\u7a33\u5b9a\u7684DN<\/span>A\u805a\u5408\u9176\uff0cPCR\u53ef\u4ee5\u7528DNA\u7684\u5408\u6210\u539f\u6599\uff0c<\/span>\u8131\u6c27\u6838\u82f7\u4e09\u78f7\u9178\u5408\u6210\u5927\u91cf\u7684DNA\u62f7\u8d1d\u3002PCR\u53cd\u5e94\u5305\u62ec\u4e09\u6b65\uff0c<\/span>\u53d8\u6027\u3001\u9000\u706b\u3001\u548c\u5ef6\u4f38\u3002<\/span>\n<\/p>\n

\n\t \n<\/p>\n

\n\t\u7167\u7247<\/span><\/strong>\n<\/p>\n

\n\tb. DNA\u7684\u63d0\u53d6,\u7535\u6cf3\u548cPCR\u64cd\u4f5c\u5b9e\u9a8c\u3002<\/span><\/strong>
\n\t\"\"\n<\/p>\n

\n\tc. \u674e\u4ee3\u82b9\u6559\u6388\u5bf9\u8df3\u86db\u7684\u751f\u6001\u7814\u7a76\u8bb2\u5ea7\u3002<\/span><\/strong>
\n\t\"\"<\/p>\n","protected":false},"excerpt":{"rendered":"

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